Anti-JunB (Phospho-Ser259) Antibody

Anti-JunB (Phospho-Ser259) Antibody

• Description: Rabbit Polyclonal Antibody specific to JunB (Phospho-Ser259)
• Product Name Alternative: Anti-JunB (Phospho-Ser259) Rabbit Polyclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: JUNB
• Gene ID: 165161
• NCBI Gene ID: JUNB
• UniProt: P17275
• Cellular Locus: Nucleus.
• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Immunogen: Peptide sequence that includes phosphorylation site of Serine 259 (P-B-S(p)-P-I) derived from human JunB and conjugated to KLH.
• Target Antigen: Transcription factor JunB
• Target: JunB (Phospho-Ser259)
• Clonality: Polyclonal
• Type: Antibody
• Applications: WB, IHC
• Field of Research: Phosphospecific Antibodies
• Purification Method: Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[GC]TCA-3'. Heterodimerizes with proteins of the FOS family to form an AP-1 transcription complex, thereby enhancing its DNA binding activity to an AP-1 consensus sequence and its transcriptional activity (By similarity). {UniProtKB:P09450}.
• Additionnal Information: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.
  
  Immunohistochemistry: use at dilution of 1:50- 1:100.
  
  These are recommended working dilutions.
  
  End user should determine optimal dilutions for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody detects endogenous human, mouse, and rat JunB only when phosphorylated at serine 259.
• Formulation: PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Buffer pH: pH 7.4
• Target Background: Transcription factor JunB is a protein that in humans is encoded by the JUNB gene and is involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5′-TGA[CG]TCA-3′.