Anti-Rel (Phospho-Ser503) Antibody

Anti-Rel (Phospho-Ser503) Antibody

• Description: Rabbit Polyclonal Antibody specific to Rel (Phospho-Ser503)
• Product Name Alternative: Anti-Rel (Phospho-Ser503) Rabbit Polyclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: REL
• Gene ID: 164910
• NCBI Gene ID: REL
• UniProt: Q04864
• Cellular Locus: Nucleus
• Host: Rabbit
• Reactivity: Human
• Immunogen: Peptide sequence that includes phosphorylation site of Serine 503 (T-S-S(p)-D-S) derived from human Rel and conjugated to KLH.
• Target Antigen: Proto-oncogene c-Rel
• Target: Rel (Phospho-Ser503)
• Clonality: Polyclonal
• Type: Antibody
• Applications: IF
• Field of Research: Phosphospecific Antibodies
• Purification Method: Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
• Additionnal Information: Immunofluorescence: use at dilution of 1:100-1:200.
  
  These are recommended working dilutions.
  
  End user should determine optimal dilutions for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody detects endogenous human Rel only when phosphorylated at serine 503.
• Formulation: PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Buffer pH: pH 7.4
• Target Background: Transcription factor c-Rel is a member of the Rel/NFkappaB family which also includes Rel-A, Rel-B, NFkappaB1, and NFkappaB2. These proteins are related through a highly conserved N-terminal region referred to as the “Rel domain†which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFkappaB inhibitor.