Anti-JunD (Phospho-Ser255) Antibody

Anti-JunD (Phospho-Ser255) Antibody

• Description: Rabbit Polyclonal Antibody specific to JunD (Phospho-Ser255)
• Product Name Alternative: Anti-JunD (Phospho-Ser255) Rabbit Polyclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: JUND
• NCBI Gene ID: JUND
• UniProt: P17535
• Cellular Locus: Nucleus.
• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Immunogen: Peptide sequence that includes phosphorylation site of serine 255 (G-E-S(p)-P-P) derived from human JunD and conjugated to KLH.
• Target Antigen: Transcription factor JunD
• Target: JunD (Phospho-Ser255)
• Clonality: Polyclonal
• Type: Antibody
• Applications: WB, IHC
• Field of Research: Phosphospecific Antibodies
• Purification Method: Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Transcription factor binding AP-1 sites (PubMed:9989505). Heterodimerizes with proteins of the FOS family to form an AP-1 transcription factor complex, thereby enhancing their DNA binding activity to an AP-1 consensus sequence 3'-TGA[GC]TCA-5' and enhancing their transcriptional activity (PubMed:9989505, PubMed:28981703). {PubMed:28981703, PubMed:9989505}.
• Additionnal Information: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~38kDa is detected.
  
  Immunohistochemistry: use at dilution of 1:50- 1:100.
  
  These are recommended working dilutions.
  
  End user should determine optimal dilutions for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody detects endogenous human, mouse, and rat JunD only when phosphorylated at serine 255.
• Formulation: PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Buffer pH: pH 7.4
• Target Background: JunD protein encoded by this intronless gene is a member of the JUN family and a functional component of the AP1 transcription factor complex. This protein has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternative translation initiation site usage results in the production of different isoforms.