Anti-GATA1 (phospho-Ser310) Antibody

Anti-GATA1 (phospho-Ser310) Antibody

• Description: Rabbit Polyclonal Antibody specific to GATA1 (phospho-Ser310)
• Product Name Alternative: Anti-GATA1 (phospho-Ser310) Rabbit Polyclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: GATA1
• Gene ID: 305371
• NCBI Gene ID: GATA1
• UniProt: P15976
• Cellular Locus: Nucleus.
• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Immunogen: Peptide sequence that includes phosphorylation site of Serine 310 (K-A-S(p)-G-K) derived from human GATA1 and conjugated to KLH.
• Target Antigen: Erythroid transcription factor
• Target: GATA1 (phospho-Ser310)
• Clonality: Polyclonal
• Type: Antibody
• Applications: WB, IF
• Field of Research: Phosphospecific Antibodies
• Purification Method: Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
• Concentration: 1.0 mg/mL
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781). {PubMed:22235304, PubMed:24245781}.
• Additionnal Information: Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~43kDa is detected.
  
  Immunofluorescence: use at dilution of 1:100-1:200.
  
  These are recommended working dilutions.
  
  End user should determine optimal dilutions for their applications
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody detects endogenous human, mouse, and rat GATA1 only when phosphorylated at serine 310.
• Formulation: PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Buffer pH: pH 7.4
• Target Background: GATA1 encodes a protein, GATA1, which belongs to the GATA family of transcription factors. This protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.