Recombinant Human B7-H4/VTCN1 Protein (Fc Tag)

Recombinant Human B7-H4/VTCN1 Protein (Fc Tag)

• Background: V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type-III membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.
• Synonyms: B7-H4, B7H4T-cell costimulatory molecule B7x, B7S1, B7S1VCTN1, B7XPRO1291, B7h.5, B7x, FLJ22418, Immune costimulatory protein B7-H4, Protein B7S1, T cell costimulatory molecule B7x, V-set domain containing T cell activation inhibitor 1, V-set domain-containing , Vtcn1
• CAS Number: 9000-83-3
• UniProt: Q7Z7D3
• Accession Number: Q7Z7D3-1
• Host: HEK293 Cells
• Origin Species: Human
• Tag: C-hFc
• Sequence: Phe29-Ala258
• Applications: Cell culture
• Field of Research: Cell Biology,Immunology,Cancer
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured by its ability to inhibit anti-CD3 antibody and anti-CD28 antibody induced IFNγ secretion in human T lymphocytes. The ED50 for this effect is typically 0.7-3. 5μg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 66-76 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.