Anti-CD98(UM7F8), CF488A conjugate

Anti-CD98(UM7F8), CF488A conjugate

• Description: CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: 4F2, 4F2HC, 4T2HC, CD98HC; MDU1; NACAE; Slc3a2; Solute Carrier Family 3 (activators of dibasic and neutral amino acid transport) Member 2, E16, LAT1, MPE16, hLAT1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SLC3A2
• Gene ID: 6520
• NCBI Gene ID: 502769
• UniProt: P08195
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Molt 13 T cell line
• Target Antigen: CD98
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: UM7F8
• Conjugation: CF488A
• Source: Animal
• Applications: Flow, surface (published) | IF (published) | Functional studies (published) | IP (published)
• Validated Applications: FC, IF, IP
• Field of Research: Transporters
• Positive Control: HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: Heterodimer (125 kDa) made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cell Immunol (1994) 154(1):253-63. (IP, Flow, functional studies) J Cell Biol (2001) 276(42): 39282-39289. (IF) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 280-283. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 531-534.
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years