Anti-CD98(UM7F8), CF640R conjugate

• Description:

  CD98 exits as a heterodimer containing a disulphide-linked glycosylated heavy chain and a non-glycosylated light chain. It is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through disulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  4F2, 4F2HC, 4T2HC, CD98HC; MDU1; NACAE; Slc3a2; Solute Carrier Family 3 (activators of dibasic and neutral amino acid transport) Member 2, E16, LAT1, MPE16, hLAT1

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SLC3A2

• Gene ID:

  6520

• NCBI Gene ID:

  502769

• UniProt:

  P08195

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Molt 13 T cell line

• Target Antigen:

  CD98

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  UM7F8

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow, surface (published) | IF (published) | Functional studies (published) | IP (published)

• Validated Applications:

  FC, IF, IP

• Field of Research:

  Transporters

• Positive Control:

  HL-60, Jurkat, MG63, HUT-78, K562, YT, U937, Hep-G2 cells, Tonsil

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  Heterodimer (125 kDa) made of a disulfide-linked glycosylated heavy chain of approximately 80-90 kDa and a non-glycosylated light chain

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Immunofluorescence 0.5-1.0 ug/mL|Comitogenic with soluble anti-CD2 and immobilized anti-CD3 mAbs|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Cell Immunol (1994) 154(1):253-63. (IP, Flow, functional studies) J Cell Biol (2001) 276(42): 39282-39289. (IF) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 280-283. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 531-534.

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years