POLR2A Antibody / RNA polymerase II subunit RPB1

POLR2A Antibody / RNA polymerase II subunit RPB1

• Description: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.
• CAS Number: 9007-83-4
• UniProt: P24928
• Host: Mouse
• Immunogen: The immunogen for this POLR2A antibody was 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-S5.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: FACS, IF, WB, IHC-P
• Format: Purified
• Buffer: 1 mg/ml in 1X PBS; BSA free, sodium azide free
• Reconstitution: Store the POLR2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This POLR2A antibody is available for research use only.