POLR2A Antibody / RNA polymerase II subunit RPB1

POLR2A Antibody / RNA polymerase II subunit RPB1

• Description: DNA-directed RNA polymerase II subunit RPB1, also known as RPB1, is an enzyme that in humans is encoded by the POLR2A gene. It is mapped to 17p13.1. This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA.
• CAS Number: 9007-83-4
• UniProt: P24928
• Host: Rabbit
• Immunogen: Recombinant human protein (amino acids D10-E321) was used as the immunogen for the POLR2A antibody.
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB, IHC-P, IF, FACS, Direct ELISA
• Format: Antigen affinity purified
• Buffer: 0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Reconstitution: After reconstitution, the POLR2A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
• Limitations: This POLR2A antibody is available for research use only.