Anti-Rt PLSCR1 Ascites

Anti-Rt PLSCR1 Ascites

• Background: PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
• Specifications: The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Rat
• Immunogen: Pooled lipid raft fraction isolated from RBL-2H3 cells
• Target Antigen: PLSCR1
• Isotype: IgG2a
• Clone: 13A6 [TEC-23]
• Applications: Western blotting: Recommended dilution: 1 μg/mL, positive control: RBL (rat basophilic leukemia) cell line both reducing and non-reducing conditions.Flow cytometry: Intracellular staining.
• Validated Applications: IP, WB, ICC, FC
• Format: Ascites
• Buffer: Stabilized with 15mM sodium azide.
• References & Citations: *Smrž D, Lebduška P, Dráberová L, Korb J, Dráber P: Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem. 2008 Apr 18283(16):10904-18., URL: https://www.ncbi.nlm.nih.gov/pubmed/18281686?ordin,
• Other References: *Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8276(23):20407-12. Epub 2001 Mar 20., URL: https://pubmed.ncbi.nlm.nih.gov/11259432/,*Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 1562(2):397-402. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11809687?dopt=,*Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep78(17):8983-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/15308695?dopt=,
• Storage Conditions: Store at 2-8°C. Do not freeze.