Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody

Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody

• Background: Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7 GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized (Chen and Dittmer 2011). In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity (Hutchinson et al., 2011). Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)–Akt–mTOR pathway (Chen and Dittmer 2011).
• Description: Boster Bio Anti-Phospho-Ribosomal Protein S6 (Ser244) Antibody (Catalog # P01567). Tested in WB applications. This antibody reacts with Human, Mouse.
• Synonyms: 40S ribosomal protein S6 antibody, Air8 antibody, NP33 antibody, Phosphoprotein NP33 antibody, Pp30 antibody, Ribosomal protein S6 antibody, RP S6 antibody, rps6 antibody, RPS6 antibody, RS6 antibody, RS6_HUMAN antibody, S6 antibody, S6 Ribosomal Protein antibody
• CAS Number: 9007-83-4
• Gene Name: RPS6
• UniProt: P62754
• Host: Rabbit
• Reactivity: Human, Mouse
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser244 of mouse rpS6, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Mouse.
• Clonality: Polyclonal
• Applications: WB
• Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Molecular Weight: 28681 MW
• Components: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Storage Conditions: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
• Fragment: IgG
• Applications Notes: Specific for endogenous levels of the ~28 kDa rpS6 protein phosphorylated at Ser244. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
• Phospho Site: Phosphorylated, Ser244