Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific

Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific

• Background: PRAS40 (proline-rich Akt/PKB substrate of 40 kDa) acts at the intersection of the Akt- and mTOR-mediated signaling pathways and its phosphorylation by Akt and by mTORC1 results in dissociation of PRAS40 from mTORC1 which may relieve an inhibitory constraint on mTORC1 activity (Wiza et al., 2012). Phosphorylation of PRAS40 by Akt and association with 14-3-3 has also been indicated to be crucial for insulin to stimulate mTOR. (Vander Haar et al., 2007). The primary function of PRAS40 in vivo in Drosophila has been shown to regulate TORC1 activity, and not to act as a downstream target and effector of TORC1 (Pallares et al., 2012).
• Description: Boster Bio Anti-Phospho-Thr356 PRAS40 Antibody, Phospho-Specific (Catalog # P03629). Tested in WB applications. This antibody reacts with Drosophila.
• Synonyms: 40 kDa proline rich AKT substrate antibody, 40 kDa proline-rich AKT substrate antibody, AKT1 S1 antibody, AKT1 substrate 1 (proline rich) antibody, AKT1 substrate 1 antibody, AKT1S 1 antibody, AKT1S1 antibody, AKTS1_HUMAN antibody, Lobe antibody, MGC2865 antibody, PRAS 40 antibody, PRAS antibody, PRAS40 antibody, Proline rich akt substrate antibody, Proline rich Akt substrate 40 kDa antibody, Proline-rich AKT1 substrate 1 antibody
• CAS Number: 9007-83-4
• Gene Name: AKT1S1
• UniProt: Q7JXA2
• Host: Rabbit
• Reactivity: Drosophila
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr356 of Drosophila PRAS40, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Drosophila.
• Clonality: Polyclonal
• Applications: WB
• Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Form: Liquid
• Molecular Weight: 27383 MW
• Components: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Storage Conditions: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
• Fragment: IgG
• Applications Notes: Specific for endogenous levels of the ~60 kDa PRAS40 protein phosphorylated at Thr356. The immunolabeling is completely eliminated by treatment with λ-phosphatase.
• Phospho Site: Phosphorylated, Thr356