Human Properdin / Complement Factor P ELISA Kit PicoKine®

Human Properdin / Complement Factor P ELISA Kit PicoKine®

• Background: Properdin or Factor P is the only known positive regulator of complement activation that stabilizes thealternative pathway convertases. This gene encodes a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Description: Human Properdin / Complement Factor P ELISA Kit PicoKine® (96 Tests). Quantitate Human CFP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Properdin; Complement factor P; CFP; PFC
• CAS Number: 7732-18-5
• Gene Name: complement factor properdin
• Gene ID: 5199
• UniProt: P27918
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D28-L469
• Applications: ELISA
• Standard: 20 ng/tube
• Sample Type: cell culture supernatants, serum and plasma (heparin, EDTA).
• Detection Range: 312 pg/ml - 20,000 pg/ml
• Sensitivity: <10 pg/ml
• : heparin or EDTA
• Function: A positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	616	576	554	549	573	26.42	4.6%
  Sample 2	2125	2256	2211	2286	2219	60.73	2.7%
  Sample 3	9062	9597	10032	10437	9782	510.91	5.2%

  *number of samples for each test n=16.
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant human Properdin
• Subcellular Location: Secreted.