Human Complement C2 ELISA Kit PicoKine®

Human Complement C2 ELISA Kit PicoKine®

• Background: Complement C2 is a protein that in humans is encoded by the C2 gene. Component C2 is a serum glycoprotein that functions as part of the classical pathway of the complement system. Activated C1 cleaves C2 into C2a and C2b. The serine proteinase C2a then combines with complement factor 4b to create the C3 or C5 convertase. Deficiency of C2 has been reported to associated with certain autoimmune diseases and SNPs in this gene have been associated with altered susceptibility to age-related macular degeneration. This gene localizes within the class III region of the MHC on the short arm of chromosome 6. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described in publications but their full-length sequence has not been determined.
• Description: Human Complement C2 ELISA Kit PicoKine® (96 Tests). Quantitate Human C2 in cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• CAS Number: 7732-18-5
• Gene Name: C2
• Gene ID: 717
• UniProt: P06681
• Reactivity: Human
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: A21-L752
• Applications: ELISA
• Standard: 20 ng/tube
• Detection Range: 312 pg/ml - 20,000 pg/ml
• : heparin, EDTA or citrate
• Sample Type: cell culture supernatants, serum, plasma(heparin, EDTA, citrate) and urine
• Sensitivity: <20 pg/ml
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	785	774	769	782	778	22.55	2.9%
  Sample 2	1654	1723	1645	1697	1680	43.67	2.6%
  Sample 3	10235	10156	10619	10665	10306	371.03	3.6%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant human C2