Anti-CDK4 Antibody (Monoclonal, DCS-31)

Anti-CDK4 Antibody (Monoclonal, DCS-31)

• Background: Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
• Description: Boster Bio Anti-CDK4 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
• CAS Number: 9007-83-4
• Gene Name: CDK4
• Gene ID: Human(1019), Mouse(12567), Rat(94201)
• UniProt: P11802
• Host: Mouse
• Reactivity: Human,Mouse,Rat
• Cross Reactivity: No cross-reactivity with other proteins
• Immunogen: Recombinant human Cdk4 protein.
• Clonality: Monoclonal
• Clone: Clone: DCS-31
• Tissue Specificity: Brain.
• Applications: ICC,WB
• Purification: Ascites
• Concentration: Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml.
• Form: Lyophilized
• Reconstitution: Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
• Function: Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .
• Components: Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• References & Citations: 1. Demetrick, D. J.; Zhang, H.; Beach, D. H. : Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes. Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Modiano, J. F.; Mayor, J.; Ball, C.; Fuentes, M. K.; Linthicum, D. S. : CDK4 expression and activity are required for cytokine responsiveness in T cells. J. Immun. 165: 6693-6702, 2000.
• Storage Conditions: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
• Calculated Molecular Weight: 44225 MW
• Observed Molecular Weight: 34 kDa
• Fragment: Mouse IgG2a
• Specificity: No cross reactivity with other proteins.
• Applications Notes: Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
  Western blot, 0.5-1μg/ml, Human, mouse, rat
  
• Subcellular Location: Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.
• Sequence Similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
• Protein Name: Cyclin-dependent kinase 4