SARS-CoV-2 Neutralization Antibody ELISA Kit

SARS-CoV-2 Neutralization Antibody ELISA Kit

• Abbreviation: SARS-CoV-2 Neutralization Antibody
• Synonyms: COVID 19 Neutralization Antibody;Anti-RBD;RBD Antibody;S1RBD Antibody;
• CAS Number: 7732-18-5
• Species Reactivity: Human
• Type: Competitive-ELISA
• Applications: ELISA
• Field of Research: SARS-CoV-2;COVID 19
• Detection Method: Colormetric
• Assay Principle: This ELISA kit uses Competitive-ELISA as the method to qualitatively detect the Anti-SARS-CoV-2 Neutralization Antibody in the sample. _x005F_x000D_ The micro ELISA plate provided in this kit is pre-coated with recombinant human ACE2. During the reaction, the SARS-CoV-2 Neutralization Antibody in the pretreated samples or controls competes with a fixed amount of human ACE2 on the solid phase supporter for sites on the Horseradish peroxidase (HRP) conjugated recombinant SARS-CoV-2 RBD fragment (HRP-RBD) . After 37°C incubation, the unbound HRP-RBD as well as any HRP-RBD bound to non-Neutralization antibody will be captured on the plate and eventually form the ACE2-RBD-HRP complex, while the circulating neutralization antibodies HRP-RBD complexes remain in the supernatant and are removed during washing. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Compared with the inhibition ratio to judge whether SARS-CoV-2 Neutralization Antibody exists in the tested samples or not.
• Assay Performance Time: 1 h 30 min
• Shipping Conditions: Ice bag
• Storage Conditions: 2-8°C, 6 months