Recombinant SARS-CoV-2 (SARS-CoV-2) S protein RBD (C-mFc-6His tag)

• Background:

  SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.

• Abbreviation:

  Spike

• Synonyms:

  COVID-19; S glycoprotein RBD; S protein RBD; Spike glycoprotein Receptor-binding domain; Spike protein RBD

• UniProt:

  P0DTC2

• Accession Number:

  QHD43416.1

• Expression System:

  HEK293 Cells

• Tag:

  C-mFc-His

• Sequence:

  Arg319-Phe541

• Applications:

  ELISA

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human S-RBD, mFc-His tagged protein (PKSV030276) can bind Anti-SARS-CoV-2 Neutralizing antibody (E-AB-V1028) in a linear range of 0.24-15.62 ng/ml. 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PKSV030276) can bind Human ACE2, hFc Tagged protein (PKSR030492) in a linear range of 0.488-49.83 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  52.2 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4.
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  SARS-CoV-2