Biotinylated Human HLA-B*07:02&B2M&HPV16 E7 (KPTLKEYVL) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-B*07:02&B2M&HPV16 E7 (KPTLKEYVL) Complex Protein (Monomer, MALS verified)

• Background: Human papillomavirus (HPV) is A kind of papillomavirus belonging to the milk polypoid virus family. It is a spherical D virus, which can cause the proliferation of squamous epithelium of human skin mucosa. HPV(human papillomavirus) for common warts, genital warts (condyloma acuminatum), and other symptoms. There are many types of human papillomavirus (HPV), with HPV 16 and 18 being high-risk types known to significantly increase the risk of cervical, vaginal and vulvar cancers in women and men. This human HLA-B*0702 HPV (KPTLKEYVL) complex protein is a complex of HLA-B*0702 of the MHC Class I, B2M, and KPTLKEYVL peptide of the HPV.
• Description: Biotinylated Human HLA-B*07:02&B2M&HPV16 E7 (KPTLKEYVL) Complex Protein (HL7-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Val 309 (HLA-B*07:02) & Ile 21 - Met 119 (B2M) & KPTLKEYVL peptide (Accession # QDK63242.1 (HLA-B*07:02) & P61769 (B2M) & KPTLKEYVL).
• Specifications: Biotinylated Human HLA-B*07:02&B2M&HPV16 E7 (KPTLKEYVL) Complex Protein is produced by co-expression of HLA and B2M loaded with HPV16 E7 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.5 kDa and 11.7 kDa. The protein migrates as 42-45 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Val 309 (HLA-B*07:02) & Ile 21 - Met 119 (B2M) & KPTLKEYVL peptide
• Host: HEK 293
• Target: HLA-B*0702 & B2M & HPV16 E7 (KPTLKEYVL)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-B*07:02&B2M&HPV16 E7 (KPTLKEYVL) Complex Protein (Cat. No. HL7-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.5 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C