Biotinylated Human HLA-C*07:02:01&B2M Monomer Protein (Peptide free, MALS verified)

Biotinylated Human HLA-C*07:02:01&B2M Monomer Protein (Peptide free, MALS verified)

• Background: HLA-A, B, and C are transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. The C receptor is a heterodimer consisting of a HLA-C mature gene product (heavy chain) and β2-microglobulin (light chain). The mature C chain is anchored in the membrane. HLA class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. HLA-C are expressed in nearly all cells, and present small peptides to the immune system which surveys for non-self peptides.
• Description: Biotinylated Human HLA-C*07:02:01&B2M Monomer Protein (HLM-H82Ed) is expressed from human 293 cells (HEK293). It contains AA Cys 25 - Ile 308 (HLA-C*07:02:01) & Ile 21 - Met 119 (B2M) (Accession # P10321-1 (HLA-C*07:02:01) & P61769-1 (B2M)).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.1 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Cys 25 - Ile 308 (HLA-C*07:02:01) & Ile 21 - Met 119 (B2M)
• Host: HEK 293
• Target: HLA-C*070201 & B2M
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-C*07:02:01&B2M Monomer Protein (Cat. No. HLM-H82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.1 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C