Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)

Recombinant Human GM-CSF/CSF2 Protein (His Tag, Cells)

• Background: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells; B cells; macrophages; mast cells; endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors; GM-CSF is also a growth factor for erythroid; megakaryocyte and eosinophil progenitors. On mature hematopoietic; monocytes/ macrophages and eosinophils. GM-CSF has a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally; GM-CSF can also stimulate the proliferation of a number of tumor cell lines; including osteogenic sarcoma; carcinoma and adenocarcinoma cell lines.
• Abbreviation: GM-CSF;CSF2
• Synonyms: CSF, CSF2, Colony-Stimulating Factor, GM-CSF, GMCSF, Granulocyte-Macrophage Colony-Stimulating Factor, Molgramostin, Sargramostim
• CAS Number: 9000-83-3
• UniProt: P04141
• Accession Number: P04141
• Host: HEK293 Cells
• Origin Species: Human
• Tag: C-His
• Sequence: Ala18-Glu144
• Applications: Cell culture
• Field of Research: immunology
• Purity: > 95 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 6-30 pg/ml.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 17-30 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.