Recombinant Mouse GM-CSF/CSF2 Protein

Recombinant Mouse GM-CSF/CSF2 Protein

• Background: Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
• Abbreviation: GM-CSF;CSF2
• Synonyms: Colony-stimulating factor, Csf2, Csfgm, GM-CSF, Granulocyte-macrophage colony-stimulating factor
• CAS Number: 9000-83-3
• UniProt: P01587
• Accession Number: P01587
• Host: E. coli
• Origin Species: Mouse
• Tag: N-His
• Sequence: Ala18-Lys141
• Applications: Cell culture
• Purity: > 98 % as determined by reducing SDS-PAGE.
• Bioactivity: Measure by its ability to induce proliferation in FDC-P1 cells. The ED₅₀ for this effect is <50 pg/mL. The specific activity of recombinant mouse GM-CSF is approximately >2 x 10⁷ IU/mg.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 17 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.