Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) (CDH1/2208R)
CAT:
37-BNUB2208-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-E-Cadherin (CDH1) / CD324 (Intercellular Junction Marker) (CDH1/2208R)
- Description: This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of calcium-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as beta-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: Arc 1; cadherin 1 type 1 E-cadherin; Cadherin1; CAM 120/80; CD324; CDH1; CDHE; E-Cad/CTF3; E-cadherin; ECAD; Epithelial cadherin; epithelial calcium dependent adhesion protein; Liver cell adhesion molecule (LCAM); Uvomorulin (UVO)
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: CDH1
- Gene ID: 999
- NCBI Gene ID: 461086
- UniProt: P12830
- Cellular Locus: Plasma membrane
- Host: Rabbit
- Species Reactivity: Human
- Immunogen: Recombinant full-length human E-Cadherin protein
- Target Antigen: CD324 | E-Cadherin
- Clonality: Recombinant Monoclonal
- Isotype: IgG
- Clone: CDH1/2208R
- Conjugation: Purified, with BSA
- Disease: Tumor
- Source: Animal
- Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
- Validated Applications: FC, IF, IHC, FFPE, WB
- Field of Research: Cancer, Cell adhesion
- Positive Control: MCF-7, LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
- Concentration: 0.2 mg/mL
- Buffer: PBS, 0.05% BSA, 0.05% azide
- Molecular Weight: 120-80 kDa (Mature); 135 kDa (Precursor)
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Does not react with mouse or rat|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years