Anti-dsRNA monoclonal antibody K1

Anti-dsRNA monoclonal antibody K1

• Description: Anti-dsRNA monoclonal antibody K1 can be used for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. It is recommended for Poly I:C detection (higher affinity than J2) and Anti-dsRNA monoclonal antibody J2 alternative to resolve cross-reactions and/or remove background in those rare experimental setups where J2 does not provides satisfactory results.
• Specifications: Anti-dsRNA monoclonal antibody K1 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of the sequence and nucleotide composition of the antigen. All naturally occurring dsRNAs investigated up to now (40-50 species) as well as poly(I)-poly(C) and poly(A)-poly(U) have been recognised by Anti-dsRNA monoclonal antibody K1. As described by Schönborn et al. (1991), Anti-dsRNA monoclonal antibody K1 shows higher affinity to poly(I)-poly(C) than to the other dsRNA antigens, although the difference of apparent binding constants may vary under different experimental conditions.
• Synonyms: mouse, IgG2a, kappa chain
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• UNSPSC Description: Antibodies
• HS Code: 38221900
• eClass Number: 32160702
• eClass Product Group: Antigen, Antikörper labor
• Applications: ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy.
• Form: lyophilised from a 1 mg/mLSolution in PBS
• Autoclavability: false
• Solubility: To prepare a 1 μg/μl PBS antibody solution add 200 μl (RNT-SCI-10020200) or 500 μl (RNT-SCI-10020500) sterile DNAse/RNAse-free distilled Water. As a result of the lyophilisation procedure, the reconstituted antibody may contain small amounts of denatured protein in the form of aggregates that may interfere with some applications such as immunohistochemistry (e.g. by giving high backgrounds). We therefore highly recommend to spin down (microcentrifuge) the reconstituted antibody before use and to use the supernatant only.
• Shipping Conditions: Shipped on gel packs
• Storage Conditions: Store at -20 °C to -80 °C upon reconstitution for long-term storage Avoid freeze/thaw cycles, store in aliquot. After adding 10 mM sodium azide the undiluted antibody 1 µg/µl can be stored at 4°C for a short period of time
• Shelf Life: 12 months after date of delivery