HighYield T7 sgRNA Synthesis Kit (SpCas9)
CAT:
220-RNT-105
Size:
50 Reactions
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
HighYield T7 sgRNA Synthesis Kit (SpCas9)
- Description: HighYield T7 sgRNA Synthesis Kit (SpCas9) is designed for cloning-free synthesis of SpCas9-specific single-guided RNAs (sgRNAs) via in vitro transcription. SgRNAs direct sequence-specific DNA recognition once complexed with Streptococcus pyogenes Cas9 (SpCas9)[1,2]. The resulting sgRNA/SpCas9 ribonucleoprotein (RNP) complex can thus be used for site-specific cleavage, nicking or binding of dsDNA both in vitro and in living cells depending on the choice of SpCas9 variant (e.g. wildtype, nickase (D10A), nuclease deficient(D10A/H840A)). Cleavage, nicking or binding of SpCas9 variant occurs upstream of the SpCas9-specific DNA recognition sequence 5'-NGG-3' (protospacer adjacent motif (PAM) sequence, N = any nucleotide base).
- Synonyms: Cloning-free preparation of SpCas9-specific sgRNA by in vitro transcription
- UNSPSC: 41100000
- UNSPSC Description: laboratory and scientific equipment
- HS Code: 38221900
- eClass Number: 32160405
- eClass Product Group: Biochemikalie labor, Sonstige
- Autoclavability: false
- Shipping Conditions: Shipped on gel packs
- Storage Conditions: Store at -20 °C Avoid freeze/thaw cycles
- Shelf Life: 12 months after date of delivery