Anti-Collagen Type IV

Anti-Collagen Type IV

• Description: The type IV collagen is encoded by the two genes, COL4A1 and COL4A2. It is the major structural component of basement membranes along with laminins and enactins. It is able to form insoluble fibres with high tensile strength. The Anti-Collagen Type IV Antibody can recognize type IV collagen in basement membranes in kidney, placenta, lung, skin, and cornea; but it is unable to recognize denatured type IV collagen. The antibody is useful in detecting extracellular matrix proteins as well as the loss of parts of basement membrane in carcinomas.
• CAS Number: 9007-83-4
• Reactivity: Human,Rat
• Immunogen: Purified Collagen Type IV extracted from human placenta
• Clonality: Monoclonal
• Type: Antibodies-Monoclonal
• Applications: WB,IHC,ELISA
• Concentration: 100µg/100µl
• Purity: The antibody was affinity-purified from hybridoma supernatant by affinity-chromatography.
• Buffer: PBS, pH 7.4 with 0.05% sodium azide
• Shipping Conditions: Ice Pack
• Storage Conditions: This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Fragment: IgG1, kappa light chain
• Specificity: Human collagen type IV; does not recognize denatured type IV collagen.
• Applications Notes: WB: 1:1000~1:10000 ELISA: 1:5000~1:50000 IHC: 1:50~1:500 Optimal dilutions should be calculated by the end user based on their specific application.