Anti-STAT3 (Phospho-Ser727)

Anti-STAT3 (Phospho-Ser727)

• Description: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]
• CAS Number: 9007-83-4
• Reactivity: Human,Mouse,Rat
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
• Clonality: Polyclonal
• Type: Antibodies-Polyclonal
• Applications: WB,IHC
• Concentration: 1ug/ul
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Shipping Conditions: Ice Pack
• Storage Conditions: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Specificity: STAT3 (phospho-Ser727) antibody detects endogenous levels of STAT3 only when phosphorylated at serine 727.
• Applications Notes: WB: 1:500~1:1000 IHC: 1:50~1:100