Anti-human IgE

Anti-human IgE

• Description: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.05-0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
• CAS Number: 9007-83-4
• Reactivity: Human
• Immunogen: Myelomatous human serum
• Clonality: Monoclonal
• Type: Antibodies-Monoclonal
• Applications: WB,ELISA
• Concentration: 1ug/ul
• Purity: MAbs 4F4, 5D4, E411, BE5, 4G7 and 4H10 by chromatography on protein A Sepharose MAb XTE4 by ion exchange chromatography
• Buffer: PBS, pH 7.4 with 0.05% sodium azide.
• Shipping Conditions: Ice Pack
• Storage Conditions: This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
• Fragment: IgG for MAbs BE5, 4G7 and 4H10 IgG1 for MAb XTE4 IgG2a for MAbs 4F4, 5D4 and E411
• Specificity: MAb E411 class specific for human IgE, Fc-region specific MAb 4F4 - IgE Cε3-domain specific MAb 5D4 - IgE Cε2-domain specific MAb XTE4 - epsilon chain specific Specific antibody titer is 1/1 000 000 by indirect ELISA.
• Applications Notes: Detection of total IgE level and IgE antibodies in ELISA. Recommended pairs (coating - conjugate): 5D4 – 4F4, 4G7– BE5. MAb E411 can be used for coating. MAb 4F4 can be used for IgE purification by affinity chromatography. MAb XTE4 can be used in Western blotting.