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Recombinant Mouse IL10

CAT:
171-Z200115
Size:
10 µg
Price:
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For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
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Recombinant Mouse IL10

  • Description: IL10 is produced by mouse Th2 cells following their stimulation by lectins. The main source for B cell derived IL10 in mice are Ly1 B cells that express CD5 (Ly1) and CD11. In humans IL10 is produced by activated CD8(+) peripheral blood T cells, by T helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human IL10 shows 73% amino acid homology with mouse IL10. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of, Th1 and Th2 cells.
  • CAS Number: 9000-83-3
  • Gene Name: IL10
  • Gene Aliases: IL-10, Cytokine synthesis inhibitory factor, CSIF
    Recombinant Mouse Interleukin-10 (IL10)
  • Gene ID: 16153
  • Accession Number: P18893
  • Organism: Mouse
  • Source: E. coli
  • Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method
  • Purity: >95% as determined by SDS-PAGE
  • Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
  • Function: The ED50 was determined by the dose-dependent (with IL-4) proliferation of murine MC/9 cells and was found to be &lt; 2.0 ng/mL
  • Molecular Weight: 19
  • Shipping Conditions: Ambient Temperature
  • Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
  • Appearance: Lyophilized Powder
  • Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0)