Recombinant Human MCP-2 (CCL8)

Recombinant Human MCP-2 (CCL8)

• Description: Monocyte chemotactic protein MCP-2 is a C-C chemokine. It shares over 60% amino acid identity with MCP-1 and MCP- 3 and has about 30% identity with other C-C chemokines MIP-1 alpha, RANTES, and MIP-1 beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells, and its spectrum of action on leukocytes is similar to that of MCP-3, including monocytes, T lymphocytes, natural killer cells, basophils, mast cells, and eosinophils, but differs from MCP-1, which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP- 1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.
• CAS Number: 9000-83-3
• Gene Name: CCL8
• Gene Aliases: Monocyte Chemotactic Protein-2, MCP2, CCL8, HC14, C-C motif chemokine 8
  Recombinant Human Monocyte Chemoattractant Protein-2 (CCL8)
• Gene ID: 6355
• Accession Number: P80075
• Organism: Human
• Source: E. coli
• Concentration: <1.0 EU/μg of recombinant protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE
• Solubility: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Function: The ED(50) was determined by the dose-dependent chemo-attraction of THP1 leukemic cells and was found to be ≤ 50 ng/mL, corresponding to a specific activity of &gt; 5.0 x 10^4 units/mg.
• Molecular Weight: 9
• Shipping Conditions: Ambient Temperature
• Storage Conditions: The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
• Appearance: Lyophilized Powder
• Formulation: Lyophilized from a 0.2 μm filtered 20 mM solution in PBS