Anti-TGF-beta(1D11.16.8), CF647 conjugate

Anti-TGF-beta(1D11.16.8), CF647 conjugate

• Description: This MAb recognizes TGF beta 1, 2 and 3. Three TGF-betas have been identified in mammals. TGF-beta1, TGF-beta2 and TGF-beta3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules. Biologically active TGF-beta requires dimerization of the monomers (usually homodimers) and release of the latent peptide portion. Overall, the mature region of the TGF-beta3 protein has approximately 80% identity to the mature region of both TGF-beta1 and TGF-beta2. However, the NH2 terminals or precursor regions of their molecules share only 27% sequence identity. TGF-beta's inhibit the growth of epithelial cells and stimulate the growth of mesenchymal cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: GP40; Leu9; p41; T-cell leukemia antigen; T-cell surface antigen Leu-9; Tp40; TP41
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TGFB1
• Gene ID: 7040 (beta1), 7042 (beta2) & 7043 (beta3)
• NCBI Gene ID: 645227
• UniProt: P01137 (beta1), P10600 (beta2); P61812 (beta3)
• Cellular Locus: Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Cow, Dog, Hamster, Human, Monkey, Mouse, Rat
• Immunogen: Bovine TGF-beta2 protein
• Target Antigen: TGF-Beta
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 1D11.16.8
• Conjugation: CF647
• Source: Animal
• Applications: Functional studies (published) | IHC, FFPE (published)
• Validated Applications: IHC, FFPE
• Field of Research: Signal transduction
• Positive Control: Salivary gland carcinoma
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: ~13 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Cytokine neutralization or functional assay, order Ab without BSA/azide|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Biol Reproduct (1992) 46: 561-572. (IHC, FFPE) PLOS ONE 10(7): e0132786. (functional studies)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years