Anti-Smooth Muscle Actin(ACTA2/791)
CAT:
37-BNUB0791-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No










Anti-Smooth Muscle Actin(ACTA2/791)
- Description: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: ACTA2
- Gene ID: 59
- NCBI Gene ID: 500483
- UniProt: P62736
- Cellular Locus: Cytoskeleton
- Host: Mouse
- Species Reactivity: Human, Rat
- Immunogen: Recombinant full-length human ACTA2 protein
- Target Antigen: Smooth Muscle Actin
- Clonality: Monoclonal
- Isotype: IgG2a κ
- Clone: ACTA2/791
- Conjugation: Purified, with BSA
- Source: Animal
- Applications: IF (verified) | IHC, FFPE (verified)
- Validated Applications: IF, IHC, FFPE
- Field of Research: Cytoskeleton
- Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
- Concentration: 0.2 mg/mL
- Buffer: PBS, 0.05% BSA, 0.05% azide
- Molecular Weight: 42 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Predicted to show broad reactivity|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years