Anti-Smooth Muscle Actin(1A4)

Anti-Smooth Muscle Actin(1A4)

• Description: Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ACTA2|Actin Alpha 2 Smooth Muscle Aorta|Actin Alpha Smooth Muscle|Actin Aortic Smooth Muscle|Actin Vascular Smooth Muscle|ACTSA|ACTVS|Alpha 2 Actin|Alpha Cardiac Actin|Alpha-actin-2|ASMA|Cell Growth-inhibiting Gene 46 Protein|Growth Inhibiting Gene 46
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ACTA2
• Gene ID: 59
• NCBI Gene ID: 500483
• UniProt: P62736
• Cellular Locus: Cytoskeleton
• Host: Mouse
• Species Reactivity: Baboon, Cat, Chicken, Cow, Dog, Goat, Guinea pig, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
• Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
• Target Antigen: Smooth Muscle Actin
• Clonality: Monoclonal
• Isotype: IgG2a κ
• Clone: 1A4
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IF, IHC, FFPE
• Field of Research: Cytoskeleton
• Positive Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 42 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years