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ATP (Adenosine Triphosphate) ELISA Kit

CAT:
855-ELK7901-01
Size:
48 Tests
Price:
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  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
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ATP (Adenosine Triphosphate) ELISA Kit

  • Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ATP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ATP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP in the samples is then determined by comparing the OD of the samples to the standard curve.
  • CAS Number: 7732-18-5
  • Gene Aliases: Adenosine-5'-Triphosphate
  • Field of Research: Nutrition metabolism.
  • Assay Principle: Competitive Inhibition ELISA
  • Assay Performance Time: 2.5h
  • Standard: 1000 ng/mL
  • Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Detection Range: 15.63-1000 ng/mL
  • Sensitivity: 5.18 ng/mL

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