Anti-CD45RO(UCHL1 + T200/797), CF405S conjugate
CAT:
37-BNC041209-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No






Anti-CD45RO(UCHL1 + T200/797), CF405S conjugate
- Description: Recognizes a 180-185 kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: PTPRC
- Gene ID: 5788
- NCBI Gene ID: 654514
- UniProt: P08575
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
- Target Antigen: CD45RO
- Clonality: Monoclonal
- Isotype: IgG2a κ
- Clone: UCHL-1 + T200/797
- Conjugation: CF405S
- Disease: Tumor
- Source: Animal
- Applications: IHC, FFPE (verified) | WB (verified)
- Validated Applications: IHC, FFPE, WB
- Field of Research: Immunology, Signal transduction
- Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% BSA, 0.05% azide
- Molecular Weight: 180-185 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.25-0.5 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years