Anti-CD45 / LCA (Leucocyte Marker)(Bra55), CF568 conjugate

Anti-CD45 / LCA (Leucocyte Marker)(Bra55), CF568 conjugate

• Description: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
• Synonyms: B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PTPRC
• Gene ID: 5788
• NCBI Gene ID: 654514
• UniProt: P08575
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human PTPRC protein
• Target Antigen: CD45 | LCA
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: Bra55
• Conjugation: CF568
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified)
• Validated Applications: FC, IF, IHC, FFPE
• Field of Research: Immunology, Signal transduction
• Positive Control: Jurkat, Ramos, U-698 or GA-10 cells. Tonsil.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 180-220 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years