Anti-CD31 / PECAM-1(158-2B3), CF594 conjugate

Anti-CD31 / PECAM-1(158-2B3), CF594 conjugate

• Description: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: PECAM1
• Gene ID: 5175
• NCBI Gene ID: 376675 & 514412
• UniProt: P16284
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Stimulated human leukocytes (Workshop VI)
• Target Antigen: CD31 | PECAM-1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 158-2B3
• Conjugation: CF594
• Source: Animal
• Applications: Flow (verified) | Flow, surface (published) | IF (published) | IF (verified)
• Validated Applications: FC, FC, IF, IF
• Field of Research: Angiogenesis, Cancer
• Positive Control: Jurkat cells. Tonsil or Angiosarcoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: ~100 kDa (endothelium) and ~130 kDa (platelets)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Kishimoto, T, et al. (Eds) Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth International Workshop and Conference held in Kobe, Japan, 10-14 November 1996. J Immunol (1999) 163: 553-557. (IF; Flow, surface) Blood (2002) 100(10): 3597-3603. (IF)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years