Anti-bcl-x(2H12), CF640R conjugate
CAT:
37-BNC400751-500
Size:
500 µL
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-bcl-x(2H12), CF640R conjugate
- Description: Bcl-x belongs to the Bcl-2 family of proteins and plays a crucial role in apoptosis. Bcl-X has two isoforms: Bcl-XL (long), a 26 kDa protein which suppresses cell death, and Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2 and promotes apoptosis. This MAb reacts with both Bcl-XS and Bcl-XL proteins, and shows no cross-reaction with Bcl-2 or Bax proteins._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
- Synonyms: Bcl-x; Bcl-2-like protein 1; BCL2L1; Apoptosis regulator Bcl-X
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: BCL2L1
- Gene ID: 598
- NCBI Gene ID: 516966
- UniProt: Q07817
- Cellular Locus: Cytoplasmic|Endoplasmic reticulum|Mitochondria
- Host: Mouse
- Species Reactivity: Human, Mouse, Pig, Rat
- Immunogen: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein.
- Target Antigen: Bcl-x
- Clonality: Monoclonal
- Isotype: IgG2a
- Clone: 2H12
- Conjugation: CF640R
- Source: Animal
- Applications: IHC, FFPE (verified) | WB (verified)
- Validated Applications: IHC, FFPE, WB
- Field of Research: Apoptosis, Cancer
- Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin's lymphoma.
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% BSA, 0.05% azide
- Molecular Weight: 27 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years