Anti-CD63 (Late Endosomes Marker)(LAMP3/529)
CAT:
37-BNUB0529-500
Size:
500 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-CD63 (Late Endosomes Marker)(LAMP3/529)
- Description: This MAb recognizes protein of 26 kDa-60 kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/803 or LAMP3/968 or NKI/C3 or MX-49.129.5. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression._x000D_ _x000D_
- Synonyms: gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: CD63
- Gene ID: 967
- NCBI Gene ID: 445570
- UniProt: P08962
- Cellular Locus: Exosomes/EVs|Lysosomes|Plasma membrane|Membrane/vesicular|Multivesicular bodies
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Recombinant human CD63 protein
- Target Antigen: CD63
- Clonality: Monoclonal
- Isotype: IgG2a κ
- Clone: LAMP3/529
- Conjugation: Purified, with BSA
- Disease: Tumor
- Source: Animal
- Applications: Flow (verified)
- Validated Applications: FC
- Field of Research: Cancer, Immunology
- Positive Control: U87MG, SK-MEL-28, HL60, THP-1, NIH/3T3, or MCF-7 cells. Human melanoma, spleen or lymphoma tissue.
- Concentration: 0.2 mg/mL
- Buffer: PBS, 0.05% BSA, 0.05% azide
- Molecular Weight: 26 kDa (core protein); 30-60 kDa (glycosylated)
- Additionnal Information: Flow cytometry: 1-2 ug/million cells|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|ELISA: For coating order antibody without BSA|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years