Anti-FOLR2 Alexa Fluor® 488

Anti-FOLR2 Alexa Fluor® 488

• Background: FOLR2 (folate receptor beta) is a cell surface protein that was originally thought to be specific for placenta, but it can be also expressed in other tissues, including hematopoietic cells. Its expression is increased in malignant tissues. FOLR2 may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients. FOLR2 is a marker for macrophages generated in the presence of M-CSF (M2), including M2-like tumor-associated macrophages, which exert potent immunosuppressive functions within the tumor environment, but not GM-CSF (M1), and whose expression correlates with increased folate uptake ability.
• Specifications: The mouse monoclonal antibody EM-35 recognizes an extracellular epitope on FOLR2, a 30-40 kDa cell surface protein serving as a receptor for folic acid.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Negative Species: Mouse
• Immunogen: BW5147alpha,beta- cells
• Target Antigen: FOLR2
• Isotype: IgG1
• Clone: EM-35
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL.
• Validated Applications: ICC, FC
• Concentration: 0.1 mg/mL
• Format: Alexa Fluor® 488
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Machacek C, Supper V, Leksa V, Mitulovic G, Spittler A, Drbal K, Suchanek M, Ohradanova-Repic A, Stockinger H: Folate Receptor 946 Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen. J Immunol. 2016 Sep 15197(6):2229-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/27534550,*Ohradanova-Repic A, Machacek C, Charvet C, Lager F, Le Roux D, Platzer R, Leksa V, Mitulovic G, Burkard TR, Zlabinger GJ, Fischer MB, Feuillet V, Renault G, Blüml S, Benko M, Suchanek M, Huppa JB, Matsuyama T, Cavaco-Paulo A, Bismuth G, Stockinger H: Extracellular Purine Metabolism Is the Switchboard of Immunosuppressive Macrophages and a Novel Target to Treat Diseases With Macrophage Imbalances. Front Immunol. 2018 Apr 279:852., URL: https://www.ncbi.nlm.nih.gov/pubmed/29780382 ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.