Lambda Light Chain Mouse Monoclonal Antibody [A8G3]

Lambda Light Chain Mouse Monoclonal Antibody [A8G3]

• Description: While benign (reactive) B-lymphocytic populations produce Ig-molecules containing an almost equal amount of kappa and lambda light chains, i.e. the number of cells producing kappa is more or less equal to the number producing lambda, neoplastic B-lymphocytic populations has light chain restriction (i.e., are monoclonal) producing either kappa or lambda. Consequently, demonstration of light chains is the most important procedure in the diagnosis of neoplasms of B-lymphocytes (lymphomas and leukemias). The amount of Ig produced in individual types of B-lymphocytic neoplasia vary and is often small, demanding a sensitive technique for detection. About 80% of non-Hodgkin lymphomas are B-cell lymphomas, of which the large majority express surface (s) IgM, although the expression in small cell lymphoma/chronic lymphocytic leukaemia is weak. Plasmacytoma/multiple myeloma do not show sIgM. Lymphoplasmacytic lymphoma most often shows sIgM, while plasmacytoma/multiple myeloma show sIgG in 50% and sIgA in 20%. Light chain restriction is the single most important marker for neoplasms of B-lymphocytic origin. Demonstration of heavy chains can sometime be of aid in the study of malignant lymphomas, as lymphoplasmacytic lymphomas are usually focally IgM positive, while plasmacytoma/multiple myeloma in most cases express strong cytoplasmic IgG or IgA. Precursor B-cell neoplasms are are Ig negative. Normal tonsil is appropriate control tissue: approximately 50% of the mantle zone B-cells should show a distinct membrane staining reaction, while the rest should be unstained.
• Product Name Alternative: Constant region of lambda light chains antibody Ig lambda chain C regions antibody IGLC antibody Immunoglobulin lambda constant 1 antibody Mcg marker antibody
• Abbreviation: IGLC1
• CAS Number: 9007-83-4
• Swiss Prot: P0CG04 Human
• Cellular Locus: Secreted, Cell membrane.
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Constant region of natural protein (constant region of light chain lambda chain).
• Isotype: IgG2b
• Conjugation: Non-conjugated
• Type: Mouse monoclonal Antibody
• Applications: IHC-P, WB, IF-Cell, FC
• Positive Control: Human small intestine tissue lysates, human plasma lysates, human tonsil tissue, Ramos.
• Concentration: 2 mg/mL
• Dilution: IHC-P: 1:4,000 ;WB: 1:1,000 ;IF-Cell: 1:200 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 11 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No