Canine Fc gamma RI / CD64 Protein, His Tag (MALS & SPR verified)

Canine Fc gamma RI / CD64 Protein, His Tag (MALS & SPR verified)

• Background: Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells.
• Description: Canine CD64, His Tag (FCA-C52H8) is expressed from human 293 cells (HEK293). It contains AA Gln 16 - Pro 288 (Accession # XP_025308990.1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 32.4 kDa. The protein migrates as 36-46 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gln 16 - Pro 288
• Host: HEK 293
• Target: Fc gamma RI / CD64
• Conjugation: Unconjugated
• Tag: C-10×His
• Source: Canine
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 32.4 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C