Biotinylated Human Syndecan-1 Protein, Fc,Avitag™

Biotinylated Human Syndecan-1 Protein, Fc,Avitag™

• Background: Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138, is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 / SDC1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells, but only if the cells tested are already known to be derived from blood.
• Description: Biotinylated Human Syndecan-1, Fc,Avitag (SY1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Gly 254 (Accession # P18827-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 52.0 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.
• Expression Region: Gln 23 - Gly 254
• Host: HEK 293
• Target: Syndecan-1
• Conjugation: Biotin-labeled
• Tag: C-Fc & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human Syndecan-1, Fc,Avitag (Cat. No. SY1-H82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) (0.5 μg/well) plate. can bind Anti-Syndecan-1 antibody, Mouse IgG1 with a linear range of 0.2-6 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 52.0 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C