Anti-FLASH Antibody

Anti-FLASH Antibody

• Description: Rabbit Polyclonal Antibody specific to FLASH (CT)
• Product Name Alternative: FLICE-associated huge protein
• CAS Number: 9007-83-4
• Gene Name: CASP8AP2
• Gene ID: 9994
• NCBI Gene ID: CASP8AP2
• UniProt: Q9UKL3
• Accession Number: NP_036247
• Cellular Locus: Cytoplasm. Nucleus. Nucleus, PML body. Mitochondrion. Note=Exported from the nucleus to the mitochondria upon FAS activation.
• Host: Rabbit
• Reactivity: Human, Mouse
• Immunogen: Peptide corresponding to aa 1253-1268 of human FLASH (accession no. AF165161). This sequence differs by one amino acid from that of mouse.
• Target Antigen: CASP8-associated protein 2
• Target: FLASH
• Clonality: Polyclonal
• Isotype: IgG
• Type: Antibody
• Applications: ELISA, WB, ICC, IF
• Field of Research: Apoptosis
• Purification Method: Purified by peptide immuno-affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium which is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase. {PubMed:12477726, PubMed:15698540, PubMed:17003125, PubMed:17245429}.
• Additionnal Information: Immunoblotting: use at 0.5ug/mL.
  
  Immunocytochemistry: use at 10ug/mL.
  
  Positive control: HeLa cell lysate.
  
  These are recommended concentrations.
  
  Enduser should determine optimal concentrations for their applications.
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
• Specificity: This antibody recognizes human and mouse FLASH (220kDa).
• Formulation: PBS, pH 7.4.
• Buffer pH: pH 7.4
• Target Background: A mammalian homologue of CED-4 has been identified and designated FLASH (FLICE-associated huge protein). FLASH is involved in Fas-induced apoptosis. FLASH interacts with the DEDs of caspase-8 and FADD through the DED-like domain of FLASH, resulting in activation of caspase-8. FLASH is expressed in multiple tissues and cell types.