Anti-Hu CD294 APC

Anti-Hu CD294 APC

• Background: CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.
• Specifications: The rat monoclonal antibody BM16 recognizes an extracellular epitope of CD294 / CRTH2 (prostaglandin D2 receptor 2), a G-protein-coupled seven-transmembrane protein expressed on Th2 cells, peripheral blood basophils and eosinophils.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Human
• Immunogen: Human CD294 transfected rat cell line TART/B19-12.10
• Target Antigen: CD294
• Isotype: IgG2a
• Clone: BM16
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *De Fanis U, Mori F, Kurnat RJ, Lee WK, Bova M, Adkinson NF, Casolaro V: GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007 May 15109(10):4343-50. Epub 2007 Jan 18., URL: https://pubmed.ncbi.nlm.nih.gov/17234745/,*Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, te Velde AA, Fokkens WJ, van Drunen CM, Spits H: The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012 Oct 1937(4):649-59., URL: https://pubmed.ncbi.nlm.nih.gov/23063330/,*Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Türeci Ö, Grabbe S, Taube C, Saloga J: Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol. 2012 Dec130(6):1384-93. , URL: http://www.ncbi.nlm.nih.gov/pubmed/23102918 ,*Nagata K, Tanaka K, Ogawa K, Kemmotsu K, Imai T, Yoshie O, Abe H, Tada K, Nakamura M, Sugamura K, Takano S: Selective expression of a novel surface molecule by human Th2 cells in vivo. J Immunol. 1999 Feb 1162(3):1278-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/9973380,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.