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Anti-Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker)(CCND1/3548)

CAT:
37-BNUM3548-50
Size:
50 µL
Price:
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For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
Anti-Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker)(CCND1/3548) - image 1
Anti-Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker)(CCND1/3548) - image 2
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Anti-Cyclin D1 (G1-Cyclin & Mantle Cell Lymphoma Marker)(CCND1/3548)

  • Description: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Synonyms: B cell CLL/lymphoma 1; B cell leukemia 1; B-cell lymphoma 1 protein; BCL-1 oncogene; CCND1 protein; CCND1/FSTL3 fusion gene; CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene; CCND1/PTH fusion gene; G1/S-specific cyclin-D1; Parathyroid adenomatosis 1; PRAD1 oncogene
  • CAS Number: 9007-83-4
  • UNSPSC: 41116161
  • UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
  • Gene Name: CCND1
  • Gene ID: 595
  • NCBI Gene ID: 523852; 667996
  • UniProt: P24385
  • Cellular Locus: Nucleus
  • Host: Mouse
  • Species Reactivity: Human
  • Immunogen: Recombinant fragment of human CCND1 (around aa 115-270) protein (exact sequence is proprietary)
  • Target Antigen: Cyclin D1
  • Clonality: Monoclonal
  • Isotype: IgG2b κ
  • Clone: CCND1/3548
  • Conjugation: Purified, BSA-free
  • Source: Animal
  • Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
  • Concentration: 1 mg/mL
  • Buffer: PBS, no BSA, no azide
  • Molecular Weight: 36 kDa
  • Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
  • Shipping Conditions: Room temperature
  • Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
  • Shelf Life: 2 years

Datasheet

MSDS