Anti-ORC1(7F6/1), CF594 conjugate

Anti-ORC1(7F6/1), CF594 conjugate

• Description: The initiation of DNA replication is a multi-step process that depends on the formation of pre-replication complexes, which trigger initiation. Among the proteins required for establishing these complexes are the origin recognition complex (ORC) proteins. ORC proteins bind specifically to origins of replication where they serve as scaffold for the assembly of additional initiation factors. Human ORC subunits 1-6 are expressed in the nucleus of proliferating cells and tissues, such as the testis. ORC1 and ORC2 are both expressed at equivalent concentrations throughout the cell cycle; however, only ORC2 remains stably bound to chromatin. ORC4 and ORC6 are also expressed constantly throughout the cell cycle. ORC2, ORC3, ORC4 and ORC5 form a core complex upon which ORC6 and ORC1 assemble. The formation of this core complex suggests that ORC proteins play a crucial role in the G1-S transition in mammalian cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: HSORC1; MmORC1; Origin Recognition Complex 1 (ORC1); Origin recognition complex subunit 1 (yeast homolog) like (ORC1L); Origin recognition complex subunit 1 like (S. cerevisia; Origin recognition complex subunit 1 like; PARC1; Replication control protein 1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ORC1L
• Gene ID: 4998
• NCBI Gene ID: 17908
• UniProt: Q13415
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant full-length human ORC1 protein
• Target Antigen: ORC1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 7F6/1
• Conjugation: CF594
• Source: Animal
• Positive Control: HeLa cells.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 120 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years