Anti-BRCA1 (Breast Marker)(BRCA1/2986)

Anti-BRCA1 (Breast Marker)(BRCA1/2986)

• Description: BRCA1 is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: BRCA1; Breast and ovarian cancer susceptibility protein 1; Early Onset Breast Cancer 1; Protein phosphatase 1 regulatory subunit 53; RING finger protein 53
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: BRCA1
• Gene ID: 672
• NCBI Gene ID: 194143
• UniProt: P38398
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment (around aa 445-620) of human BRCA1 protein (exact sequence is proprietary)
• Target Antigen: BRCA1
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: BRCA1/2986
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Field of Research: Cancer
• Positive Control: A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 220 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years