Human Macrophage Colony Stimulating Factor Recombinant

• Background: M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B-cells and T- cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cellsin vivo. The M-CSF receptor (CD115) is identical with the proto-oncogene fms. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.
• Description: Recombinant Human M-CSF is a disulfide-linked homodimeric protein consisting of two 149 amino acid residues, and migrates as an approximately 42 kDa protein under non-reducing and as 20-21 kDa under reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human M-CSF extracellular domain was expressed inE. coli.
• UniProt: P09603
• Endotoxin: Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
• Purity: ≥ 95% by SDS-PAGE and HPLC
• Format: Lyophilized protein
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
• Shipping Conditions: Gel Packs or Dry Ice
• Storage Conditions: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
• Calculated Molecular Weight: 21 kDa
• Formulation: Recombinant CSF1 lyophilized from a 0.2 µm filtered PBS solution pH 7.4