PLPro, His-Tag (SARS-CoV-2) Recombinant

• Background: Papain-Like Protease (PLPro), also known as PLP, plays an essential role in polypeptide processing during virus replication. PLPro is a key enzyme in the sustained pathogenesis of SARS-CoV-2. It acts as a deubiquitinase that removes ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate antiviral immune responses. As a result, PLPro is an important potential target for antiviral drugs that may inhibit viral replication and simultaneously weaken dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising drug candidates that could be used to treat COVID-19 patients.
• Description: Recombinant SARS-CoV-2 papain-like protease protein (PLPro) also known as ORF1ab polyprotein, encompassing amino acids 1564-1882. This construct contains an N-terminal His-tag (6xHis) . The recombinant protein was affinity purified.
• UniProt: P0DTD1
• Tag: N-terminal His-tag
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Purity: ≥ 80%
• Format: Aqueous buffer solution.
• Shipping Conditions: -80°C
• Storage Conditions: At least 6 months at -80°C
• Calculated Molecular Weight: 37 kDa
• Formulation: 40 mM Tris-HCl, pH 8,110 mM NaCl, 2.2mM KCl, 0.04% Tween-20,3 mM DTT, 20% glycerol, and variable Imidazole.