SIGLEC1 Antibody / CD169 / Sialoadhesin

SIGLEC1 Antibody / CD169 / Sialoadhesin

• Description: Two families of mammalian lectin-like adhesion molecules, the selectins and the sialoadhesins, bind glycoconjugate ligands in a sialic acid-dependent manner. The sialic acid-binding immunoglobulin superfamily lectins, designated siglecs or sialoadhesins, are immunoglobulin superfamily members that recognize sialylated ligands. The common sialic acids of mammalian cells are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). The human Siglec-1 gene maps to chromosome 20p13 and encodes a 1,709 amino acid protein, also known as CD169. Alternative splicing of the Siglec-1 gene produces a variant, encoding a type I transmembrane protein isoform that is soluble rather than membrane-bound. Studies have shown human Siglec-1 has greater affinity for Neu5Ac over Neu5Gc. Siglec-1 is a sialic acid-binding receptor that is expressed in hemopoietic cells. It mediates local cell-cell interactions in lymphoid tissues and can be detected at contact points of macrophages with other macrophages, sinus-lining cells and reticulum cells.
• CAS Number: 9007-83-4
• UniProt: Q9ZZ2
• Host: Mouse
• Immunogen: An Fc fusion protein containing N-terminal 4 domains of human sialoadhesin was used as the immunogen for this SIGLEC1 antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: FACS, IF, WB, IHC-P
• Format: Purified
• Buffer: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Reconstitution: Store the SIGLEC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This SIGLEC1 antibody is available for research use only.